Archive for the ‘column chromatography’ Category
The top margins of separation
During an experiment in lab class we used column chromatography to separate a mixture of phenolphthalein and bromophenol blue with 95% ethanol as the mobile phase and silica as the stationary phase. The bromophenol blue came out first, so that should mean it is less polar than the phenolphthalein as they are around the same size. Looking at the molecule though it seems the opposite.
Can anyone tell me which molecule is more polar and whether or not am I confused about how polarity affects column chromatography.
It may be that polarity is irrelevant because the polarities of the two compounds are very similar (but not the same). So you should look at bulk. Because one compound is bigger or bulkier than the other, it takes it longer to travel even though it may be less polar.
How can TLC and column chromatography be used together to ensure a perfect separation of two compounds?
no idea on this one . go to wikipedia and read
In the separation of fluorene and fluorenone during column chromatography, what is the stationary phase and what is the mobile phase?
stationary phase includes the time when the liquids are in the liquid phase, and still in solution. the mobile phase includes the time it takes to vaporize each respective compound until the end of the evaluation of the sample. the idea is to separate substances by boiling point and vapor phase.
Convenient and reliable prepacked HiTrap™ columns are designed for fast and easy small-scale preparative protein purifications. HiTrap columns can be connected in series for easy scale-up. All you need is a HiTrap column, and a syringe, pump, or a chromatography system.
Duration : 0:1:22
Paper chromatography of ink.
Column Chromatography
It takes considerable practice to master the art of “running a column”. This video will get you started, with tips on picking appropriate conditions, packing and running a column, monitoring separation, and even making a micro-column from a pipet.
Created by Dr. Sarah Tabacco and Aaeyesha Siddiqui
View the complete resource at: http://ocw.mit.edu/OcwWeb/resources/RES-5-0001Spring-2007/ResourceHome
License: Creative Commons BY-NC-SA
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WARNING NOTICE:
The experiments described in these materials are potentially hazardous and require a high level of safety training, special facilities and equipment, and supervision by appropriate individuals. You bear the sole responsibility, liability, and risk for the implementation of such safety procedures and measures. MIT shall have no responsibility, liability, or risk for the content or implementation of any of the material presented. Legal Notice – http://ocw.mit.edu/OcwWeb/web/terms/terms/index.htm
Duration : 0:22:24
Convenient and reliable prepacked HiTrap™ columns are designed for fast and easy small-scale preparative protein purifications. HiTrap columns can be connected in series for easy scale-up. All you need is a HiTrap column, and a syringe, pump, or a chromatography system.
Duration : 0:1:21
defense uyet hahaha:-) defense defense defense
Convenient and reliable prepacked HiTrap™ columns are designed for fast and easy small-scale preparative protein purifications. HiTrap columns can be connected in series for easy scale-up. All you need is a HiTrap column, and a syringe, pump, or a chromatography system.
Duration : 0:0:39