Archive for the ‘column chromatography’ Category
defense uyet hahaha:-) defense defense defense
Convenient and reliable prepacked HiTrap™ columns are designed for fast and easy small-scale preparative protein purifications. HiTrap columns can be connected in series for easy scale-up. All you need is a HiTrap column, and a syringe, pump, or a chromatography system.
Duration : 0:0:39
Convenient and reliable prepacked HiTrap™ columns are designed for fast and easy small-scale preparative protein purifications. HiTrap columns can be connected in series for easy scale-up. All you need is a HiTrap column, and a syringe, pump, or a chromatography system.
Duration : 0:0:53
Convenient and reliable prepacked HiTrap™ columns are designed for fast and easy small-scale preparative protein purifications. HiTrap columns can be connected in series for easy scale-up. All you need is a HiTrap column, and a syringe, pump, or a chromatography system.
Duration : 0:0:49
RPI Dr. Rutledge’s BIOL-4710: Biochem Lab
Thursday Group-Lee&Viola
GFP N-Terminal His Tag
Nickel Column Chromatography
Duration : 0:0:25
consider the possibilities for charge delocalization in each structure. and what would be the effect of packing a chromatographic column unevenly
do not know the answer
A 7 mix components: uracil, phenol and 5 parabenes separated on 3 mm Acclaim column, C18 3 micron particles in 53 seconds without using extreme pressure or temperature.
Duration : 0:1:4
what would be the effect on the results of a column (glass tubing) chromatography if:
A) an excessive amount of solvents were used in adding a sample to the column.
B) solvent is not maintained above the absorbent, and a crack develops in the column
A) The sample would be diluted and the components would be spread out in a larger volume when they come off the column, meaning you will have to collect a larger volume when your component of interest comes off the column. Also, it may be harder to detect since the component of interest will be diluted. Also, you will likely have to work harder to concentrate your component of interest after you collect the fractions off of the column. Also, the various components will likely overlap when coming off the column, resulting in more difficulty in finding your specific component of interest.
B) If you let the column run dry, then the components will not separate as you would expect if the column were run correctly. Also, the samples may actually remix if the cracks are large enough and the results will be useless, since the samples won’t separate based solely on the properties of the column matrix.
Is your lab looking for a better, more effective way to develop new HPLC methods? Are you tired of disconnecting columns, exchanging solvent bottles, flushing flow paths and waiting for the system to equilibrate? Heres some good news: the Agilent 1200 Series LC method development solution. Benefit from up to 288 different test conditions for binary gradient separation on a system tailored to your needs. The Agilent 1200 Series LC method development solution integrates new hardware modules and new software for system control and application-support. Agilent ZORBAX LC columns and top-rated Agilent services make this a truly one-vendor solution for all HPLC method development tasks.
http://www.agilent.com/chem/1200mds
Duration : 0:4:51