Jay Greentree | Information Technology

I have to do column chromatography to separate the Lycopene from carotene, but I don’t know what will happen if I use too much hexane for dilution.

The chromatography system can only evaporate so much sample. It you load a larger sample, the separation will not be as clean. If you load the same volume of a more dilute sample, you will get less signal and less yield.

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