Posts Tagged ‘chromatography’
RPI Dr. Rutledge’s BIOL-4710: Biochem Lab
Thursday Group-Lee&Viola
GFP N-Terminal His Tag
Nickel Column Chromatography
Duration : 0:0:25
Introducing Kinetex™ Core-Shell Technology
A leap in column particle technology that will change the way you think about UHPLC (Ultra-High Performance Liquid Chromatography). Prepare to transform the performance of your HPLC instrument and achieve UHPLC results by harnessing the power of Kinetex™.
The Kinetex core-shell particle is not fully porous. Using sol-gel processing techniques that incorporate nano-structuring technology, a durable, homogenous porous shell is grown on a solid silica core. This highly optimized growth process produces an extremely narrow particle size distribution and dramatically reduces two major sources of peak dispersion -Eddy Diffusion and Resistance to Mass Transfer. The result is a column technology engineered to reduce all major sources of band broadening that allows for ultra-high performance on any LC system.
Duration : 0:2:0
Introduction of high-quality and high-grade GC columns for gas chromatography. A direct comparison against HP and DB columns.
Duration : 0:1:7
Agilent Instructional Video: Daron Decker, GC Applications Engineer, discusses how parameters such as length of column, diameter and carrier gas affect speed and resolution, and how you can take advantage of new technologies to enhance your analyses. Part 1 and 2 discuss some of the principles of GC, for context, and parts 3 and 4 demonstrate how you can leverage Agilents Method Translator software to assist you in modifying your parameters for improved results.
Duration : 0:6:28
Agilent Instructional Video: Daron Decker, GC Applications Engineer, discusses how parameters such as length of column, diameter and carrier gas affect speed and resolution, and how you can take advantage of new technologies to enhance your analyses. Part 1 and 2 discuss some of the principles of GC, for context, and parts 3 and 4 demonstrate how you can leverage Agilents Method Translator software to assist you in modifying your parameters for improved results.
Duration : 0:9:22
Agilent Instructional Video: Daron Decker, GC Applications Engineer, discusses how parameters such as length of column, diameter and carrier gas affect speed and resolution, and how you can take advantage of new technologies to enhance your analyses. Part 1 and 2 discuss some of the principles of GC, for context, and parts 3 and 4 demonstrate how you can leverage Agilents Method Translator software to assist you in modifying your parameters for improved results.
Duration : 0:6:58
Agilent Instructional Video: Daron Decker, GC Applications Engineer, discusses how parameters such as length of column, diameter and carrier gas affect speed and resolution, and how you can take advantage of new technologies to enhance your analyses. Part 1 and 2 discuss some of the principles of GC, for context, and parts 3 and 4 demonstrate how you can leverage Agilents Method Translator software to assist you in modifying your parameters for improved results.
Duration : 0:9:55
NLISIS Meltfit One
The NLISIS Meltfit One is a new, reliable and efficient method for coupling capillary columns in GC, GCxGC, CZE and all other coupled-column capillary separation techniques.
In five simple steps the NLISIS Meltfit One establishes an automated and standardised connection between two capillary columns, using a deactivated Meltfit Tube.
Duration : 0:1:21
Hannes Binder, Ph.D. (Chief Executive Officer, Romer Labs, Tulln, Austria) answers the question: “What are official reference methods for mycotoxin analysis?”
Duration : 0:1:11
Operation Guide Video of Tauto’s High Speed Counter Current Chromatography (HSCCC) instrument
Advantages of HSCCC in isolation and purification field
Wide Range: Small molecular and big molecular compounds.
Convenient Operation: Both of the two phase are liquid. There is a wild range of liquid solvent system to choose from. It is convenient to change the solvent system.
High Degree of Separation: The APIs of the natural mixture can be isolated to the purity of 99%
Low Cost: As no column filling is used there is no need to replace the column. Only analytical grade solvents required. The cost of running and maintenance is quite low.
High Rate of Recovery : no absorptive loss and sample denaturation caused by solid adhesion.
High Throughout: The productivity of semi-preparative system can reach the gram-level and above.
Specifications
Power Source 220V±20V 50±0.5Hz
Power 200 W
Flow rate range 1.5-2.0ml/min
Column Capacity 260 ml
Sample loop 20 ml
Revolution speed range 700-900 rpm
Temperature control module Temperature range 15 ? 40 ?
Accuracy ± 0.5 ?
Pressure range 0-1Mpa
Monitor with Hg lamp 254,280nm (Standard Accessories)
Dimensions 563×638×368 mm
Main Advantages
Patented design of 3-partitioning-column and dual sixway valve;
Online detection supported.
Description
The machine adopts the design of 3 coiled column connected in series. So it can reach the perfect balance when revolving at a very high speed. The running is stable and the noise is notably low.
The separation process is swift and the operation is convenient. The balance of the machine will not be affected by changes of the density of the solvents. The stability is guaranteed.
No irreversible absorption waste.
High recovery.
Other features
It integrates a thermostatic module which can control the temperature of experiment precisely. The application range of the experiment is expanded and the repeatability is boosted. It is useful in exploring the experiment conditions at early stage. Also there are more options for choosing a suitable solvent system. It is especially suitable for separating the active ingredients sensitive to the environment temperature.
Duration : 0:9:59